Electrodeposition of Polymer Nanodots with Controlled Density and Their Reversible Functionalization by Polyhistidine-Tag Proteins
Identifieur interne : 001C93 ( Main/Repository ); précédent : 001C92; suivant : 001C94Electrodeposition of Polymer Nanodots with Controlled Density and Their Reversible Functionalization by Polyhistidine-Tag Proteins
Auteurs : RBID : Pascal:12-0425613Descripteurs français
- Pascal (Inist)
- Dépôt électrolytique, Polymère, Densité, Fonctionnalisation, Protéine, Substrat, Ion métallique, Nanoparticule, Oxyde d'indium, Oxyde d'étain, Electrode, Dispersion aqueuse, Courant continu, Microscopie force atomique, Corrélation, Particule, Ancrage, Complexation, Métal divalent, Modèle, Caractérisation, Microscopie fluorescence, Affinité, Immobilisation, Acide, EDTA.
- Wicri :
English descriptors
- KwdEn :
- Acids, Affinity, Anchoring, Aqueous dispersion, Atomic force microscopy, Characterization, Complexation, Correlation, Density, Direct current, Divalent metal, EDTA, Electrodeposition, Electrodes, Fluorescence microscopy, Functionalization, Immobilization, Indium oxide, Metal ion, Models, Nanoparticle, Particle, Polymer, Protein, Substrate, Tin oxide.
Abstract
We present a simple and rapid procedure for producing polymer-coated substrates that can be easily functionalized by ion-chelating proteins. The procedure consists of depositing 18 nm metal-chelating cyclam-modified polymer nanoparticles (cyclam-nps) onto a conductive substrate (an Indium Tin Oxide (ITO) electrode) from an aqueous dispersion of Cu2+-loaded cyclam-nps while being subjected to a direct current (DC) field. The density of deposited nps as measured by AFM is shown to be in direct correlation to the concentration of nps in the dispersion with deposition of the particles taking less than 5 s. Because of the functionalization of the nps with cyclam groups, they can be used as anchoring sites for 6-Histidine (6-His) tagged proteins through complexation with divalent metal ions. In this work 6-His Green Fluorescent Protein (6-His GFP) is used as a model protein. The characterization by fluorescence microscopy clearly shows that the protein affinity was ion dependent and that the 6-His GFP density can be controlled by np density, which is itself easily tunable. AFM observations confirmed the immobilization of 6-His GFP onto cyclam-nps and its subsequent removal by treatment with ethylenediaminetetraacetic acid (EDTA).
Links toward previous steps (curation, corpus...)
- to stream Main, to step Corpus: 001653
Links to Exploration step
Pascal:12-0425613Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Electrodeposition of Polymer Nanodots with Controlled Density and Their Reversible Functionalization by Polyhistidine-Tag Proteins</title><author><name sortKey="Bazin, Damien" uniqKey="Bazin D">Damien Bazin</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Centre de Recherche Paul Pascal- Universite de Bordeaux 1, Avenue du Dr Schweitzer</s1><s2>33600 Pessac</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Aquitaine</region><settlement type="city">Pessac</settlement></placeName></affiliation></author><author><name sortKey="Chevalier, Sebastien" uniqKey="Chevalier S">Sebastien Chevalier</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Centre de Recherche Paul Pascal- Universite de Bordeaux 1, Avenue du Dr Schweitzer</s1><s2>33600 Pessac</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Aquitaine</region><settlement type="city">Pessac</settlement></placeName></affiliation></author><author><name sortKey="Saadaoui, Hassan" uniqKey="Saadaoui H">Hassan Saadaoui</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Centre de Recherche Paul Pascal- Universite de Bordeaux 1, Avenue du Dr Schweitzer</s1><s2>33600 Pessac</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Aquitaine</region><settlement type="city">Pessac</settlement></placeName></affiliation></author><author><name sortKey="Santarelli, Xavier" uniqKey="Santarelli X">Xavier Santarelli</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Université de Bordeaux, BPRVS, EA 4135</s1><s2>33000 Bordeaux</s2><s3>FRA</s3><sZ>4 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName><orgName type="university">Université de Bordeaux</orgName></affiliation></author><author><name sortKey="Larpent, Chantal" uniqKey="Larpent C">Chantal Larpent</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Institut Lavoisier, UMR CNRS 8180, Université de Versailles St Quentin en Y., 45 Avenue des Etats Unis</s1><s2>78035 Versailles</s2><s3>FRA</s3><sZ>5 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Feracci, Helene" uniqKey="Feracci H">Hélène Feracci</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Centre de Recherche Paul Pascal- Universite de Bordeaux 1, Avenue du Dr Schweitzer</s1><s2>33600 Pessac</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Aquitaine</region><settlement type="city">Pessac</settlement></placeName></affiliation></author><author><name sortKey="Faure, Chrystel" uniqKey="Faure C">Chrystel Faure</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Centre de Recherche Paul Pascal- Universite de Bordeaux 1, Avenue du Dr Schweitzer</s1><s2>33600 Pessac</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Aquitaine</region><settlement type="city">Pessac</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="inist">12-0425613</idno><date when="2012">2012</date><idno type="stanalyst">PASCAL 12-0425613 INIST</idno><idno type="RBID">Pascal:12-0425613</idno><idno type="wicri:Area/Main/Corpus">001653</idno><idno type="wicri:Area/Main/Repository">001C93</idno></publicationStmt><seriesStmt><idno type="ISSN">0743-7463</idno><title level="j" type="abbreviated">Langmuir</title><title level="j" type="main">Langmuir</title></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acids</term><term>Affinity</term><term>Anchoring</term><term>Aqueous dispersion</term><term>Atomic force microscopy</term><term>Characterization</term><term>Complexation</term><term>Correlation</term><term>Density</term><term>Direct current</term><term>Divalent metal</term><term>EDTA</term><term>Electrodeposition</term><term>Electrodes</term><term>Fluorescence microscopy</term><term>Functionalization</term><term>Immobilization</term><term>Indium oxide</term><term>Metal ion</term><term>Models</term><term>Nanoparticle</term><term>Particle</term><term>Polymer</term><term>Protein</term><term>Substrate</term><term>Tin oxide</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Dépôt électrolytique</term><term>Polymère</term><term>Densité</term><term>Fonctionnalisation</term><term>Protéine</term><term>Substrat</term><term>Ion métallique</term><term>Nanoparticule</term><term>Oxyde d'indium</term><term>Oxyde d'étain</term><term>Electrode</term><term>Dispersion aqueuse</term><term>Courant continu</term><term>Microscopie force atomique</term><term>Corrélation</term><term>Particule</term><term>Ancrage</term><term>Complexation</term><term>Métal divalent</term><term>Modèle</term><term>Caractérisation</term><term>Microscopie fluorescence</term><term>Affinité</term><term>Immobilisation</term><term>Acide</term><term>EDTA</term></keywords><keywords scheme="Wicri" type="concept" xml:lang="fr"><term>Polymère</term><term>Acide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We present a simple and rapid procedure for producing polymer-coated substrates that can be easily functionalized by ion-chelating proteins. The procedure consists of depositing 18 nm metal-chelating cyclam-modified polymer nanoparticles (cyclam-nps) onto a conductive substrate (an Indium Tin Oxide (ITO) electrode) from an aqueous dispersion of Cu<sup>2+</sup>-loaded cyclam-nps while being subjected to a direct current (DC) field. The density of deposited nps as measured by AFM is shown to be in direct correlation to the concentration of nps in the dispersion with deposition of the particles taking less than 5 s. Because of the functionalization of the nps with cyclam groups, they can be used as anchoring sites for 6-Histidine (6-His) tagged proteins through complexation with divalent metal ions. In this work 6-His Green Fluorescent Protein (6-His GFP) is used as a model protein. The characterization by fluorescence microscopy clearly shows that the protein affinity was ion dependent and that the 6-His GFP density can be controlled by np density, which is itself easily tunable. AFM observations confirmed the immobilization of 6-His GFP onto cyclam-nps and its subsequent removal by treatment with ethylenediaminetetraacetic acid (EDTA).</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0743-7463</s0></fA01><fA02 i1="01"><s0>LANGD5</s0></fA02><fA03 i2="1"><s0>Langmuir</s0></fA03><fA05><s2>28</s2></fA05><fA06><s2>39</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Electrodeposition of Polymer Nanodots with Controlled Density and Their Reversible Functionalization by Polyhistidine-Tag Proteins</s1></fA08><fA11 i1="01" i2="1"><s1>BAZIN (Damien)</s1></fA11><fA11 i1="02" i2="1"><s1>CHEVALIER (Sebastien)</s1></fA11><fA11 i1="03" i2="1"><s1>SAADAOUI (Hassan)</s1></fA11><fA11 i1="04" i2="1"><s1>SANTARELLI (Xavier)</s1></fA11><fA11 i1="05" i2="1"><s1>LARPENT (Chantal)</s1></fA11><fA11 i1="06" i2="1"><s1>FERACCI (Hélène)</s1></fA11><fA11 i1="07" i2="1"><s1>FAURE (Chrystel)</s1></fA11><fA14 i1="01"><s1>Centre de Recherche Paul Pascal- Universite de Bordeaux 1, Avenue du Dr Schweitzer</s1><s2>33600 Pessac</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></fA14><fA14 i1="02"><s1>Institut Lavoisier, UMR CNRS 8180, Université de Versailles St Quentin en Y., 45 Avenue des Etats Unis</s1><s2>78035 Versailles</s2><s3>FRA</s3><sZ>5 aut.</sZ></fA14><fA14 i1="03"><s1>Université de Bordeaux, BPRVS, EA 4135</s1><s2>33000 Bordeaux</s2><s3>FRA</s3><sZ>4 aut.</sZ></fA14><fA20><s1>13968-13975</s1></fA20><fA21><s1>2012</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>20642</s2><s5>354000502053510270</s5></fA43><fA44><s0>0000</s0><s1>© 2012 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>43 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>12-0425613</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Langmuir</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>We present a simple and rapid procedure for producing polymer-coated substrates that can be easily functionalized by ion-chelating proteins. The procedure consists of depositing 18 nm metal-chelating cyclam-modified polymer nanoparticles (cyclam-nps) onto a conductive substrate (an Indium Tin Oxide (ITO) electrode) from an aqueous dispersion of Cu<sup>2+</sup>-loaded cyclam-nps while being subjected to a direct current (DC) field. The density of deposited nps as measured by AFM is shown to be in direct correlation to the concentration of nps in the dispersion with deposition of the particles taking less than 5 s. Because of the functionalization of the nps with cyclam groups, they can be used as anchoring sites for 6-Histidine (6-His) tagged proteins through complexation with divalent metal ions. In this work 6-His Green Fluorescent Protein (6-His GFP) is used as a model protein. The characterization by fluorescence microscopy clearly shows that the protein affinity was ion dependent and that the 6-His GFP density can be controlled by np density, which is itself easily tunable. AFM observations confirmed the immobilization of 6-His GFP onto cyclam-nps and its subsequent removal by treatment with ethylenediaminetetraacetic acid (EDTA).</s0></fC01><fC02 i1="01" i2="X"><s0>001C01J02</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Dépôt électrolytique</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Electrodeposition</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Depósito electrolítico</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Polymère</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Polymer</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Polímero</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Densité</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Density</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Densidad</s0><s5>03</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Fonctionnalisation</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Functionalization</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Funciónalización</s0><s5>04</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Protéine</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Protein</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Proteína</s0><s5>05</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Substrat</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Substrate</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Substrato</s0><s5>06</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Ion métallique</s0><s5>07</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Metal ion</s0><s5>07</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Ión metálico</s0><s5>07</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Nanoparticule</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Nanoparticle</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Nanopartícula</s0><s5>08</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Oxyde d'indium</s0><s5>09</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Indium oxide</s0><s5>09</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Indio óxido</s0><s5>09</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Oxyde d'étain</s0><s5>10</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Tin oxide</s0><s5>10</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Estaño óxido</s0><s5>10</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>Electrode</s0><s5>11</s5></fC03><fC03 i1="11" i2="X" l="ENG"><s0>Electrodes</s0><s5>11</s5></fC03><fC03 i1="11" i2="X" l="SPA"><s0>Electrodo</s0><s5>11</s5></fC03><fC03 i1="12" i2="X" l="FRE"><s0>Dispersion aqueuse</s0><s5>12</s5></fC03><fC03 i1="12" i2="X" l="ENG"><s0>Aqueous dispersion</s0><s5>12</s5></fC03><fC03 i1="12" i2="X" l="SPA"><s0>Dispersión acuosa</s0><s5>12</s5></fC03><fC03 i1="13" i2="X" l="FRE"><s0>Courant continu</s0><s5>13</s5></fC03><fC03 i1="13" i2="X" l="ENG"><s0>Direct current</s0><s5>13</s5></fC03><fC03 i1="13" i2="X" l="SPA"><s0>Corriente contínua</s0><s5>13</s5></fC03><fC03 i1="14" i2="X" l="FRE"><s0>Microscopie force atomique</s0><s5>14</s5></fC03><fC03 i1="14" i2="X" l="ENG"><s0>Atomic force microscopy</s0><s5>14</s5></fC03><fC03 i1="14" i2="X" l="SPA"><s0>Microscopía fuerza atómica</s0><s5>14</s5></fC03><fC03 i1="15" i2="X" l="FRE"><s0>Corrélation</s0><s5>15</s5></fC03><fC03 i1="15" i2="X" l="ENG"><s0>Correlation</s0><s5>15</s5></fC03><fC03 i1="15" i2="X" l="SPA"><s0>Correlación</s0><s5>15</s5></fC03><fC03 i1="16" i2="X" l="FRE"><s0>Particule</s0><s2>FX</s2><s5>16</s5></fC03><fC03 i1="16" i2="X" l="ENG"><s0>Particle</s0><s2>FX</s2><s5>16</s5></fC03><fC03 i1="16" i2="X" l="SPA"><s0>Partícula</s0><s2>FX</s2><s5>16</s5></fC03><fC03 i1="17" i2="X" l="FRE"><s0>Ancrage</s0><s5>17</s5></fC03><fC03 i1="17" i2="X" l="ENG"><s0>Anchoring</s0><s5>17</s5></fC03><fC03 i1="17" i2="X" l="SPA"><s0>Anclaje</s0><s5>17</s5></fC03><fC03 i1="18" i2="X" l="FRE"><s0>Complexation</s0><s5>18</s5></fC03><fC03 i1="18" i2="X" l="ENG"><s0>Complexation</s0><s5>18</s5></fC03><fC03 i1="18" i2="X" l="SPA"><s0>Complexación</s0><s5>18</s5></fC03><fC03 i1="19" i2="X" l="FRE"><s0>Métal divalent</s0><s2>NC</s2><s5>19</s5></fC03><fC03 i1="19" i2="X" l="ENG"><s0>Divalent metal</s0><s2>NC</s2><s5>19</s5></fC03><fC03 i1="19" i2="X" l="SPA"><s0>Metal divalente</s0><s2>NC</s2><s5>19</s5></fC03><fC03 i1="20" i2="X" l="FRE"><s0>Modèle</s0><s5>20</s5></fC03><fC03 i1="20" i2="X" l="ENG"><s0>Models</s0><s5>20</s5></fC03><fC03 i1="20" i2="X" l="SPA"><s0>Modelo</s0><s5>20</s5></fC03><fC03 i1="21" i2="X" l="FRE"><s0>Caractérisation</s0><s5>21</s5></fC03><fC03 i1="21" i2="X" l="ENG"><s0>Characterization</s0><s5>21</s5></fC03><fC03 i1="21" i2="X" l="SPA"><s0>Caracterización</s0><s5>21</s5></fC03><fC03 i1="22" i2="X" l="FRE"><s0>Microscopie fluorescence</s0><s5>22</s5></fC03><fC03 i1="22" i2="X" l="ENG"><s0>Fluorescence microscopy</s0><s5>22</s5></fC03><fC03 i1="22" i2="X" l="SPA"><s0>Microscopía fluorescencia</s0><s5>22</s5></fC03><fC03 i1="23" i2="X" l="FRE"><s0>Affinité</s0><s5>23</s5></fC03><fC03 i1="23" i2="X" l="ENG"><s0>Affinity</s0><s5>23</s5></fC03><fC03 i1="23" i2="X" l="SPA"><s0>Afinidad</s0><s5>23</s5></fC03><fC03 i1="24" i2="X" l="FRE"><s0>Immobilisation</s0><s5>24</s5></fC03><fC03 i1="24" i2="X" l="ENG"><s0>Immobilization</s0><s5>24</s5></fC03><fC03 i1="24" i2="X" l="SPA"><s0>Inmovilización</s0><s5>24</s5></fC03><fC03 i1="25" i2="X" l="FRE"><s0>Acide</s0><s5>25</s5></fC03><fC03 i1="25" i2="X" l="ENG"><s0>Acids</s0><s5>25</s5></fC03><fC03 i1="25" i2="X" l="SPA"><s0>Acido</s0><s5>25</s5></fC03><fC03 i1="26" i2="X" l="FRE"><s0>EDTA</s0><s2>NK</s2><s5>26</s5></fC03><fC03 i1="26" i2="X" l="ENG"><s0>EDTA</s0><s2>NK</s2><s5>26</s5></fC03><fC03 i1="26" i2="X" l="SPA"><s0>EDTA</s0><s2>NK</s2><s5>26</s5></fC03><fN21><s1>331</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=IndiumV3/Data/Main/Repository
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C93 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Repository/biblio.hfd -nk 001C93 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= *** parameter Area/wikiCode missing ***
|area= IndiumV3
|flux= Main
|étape= Repository
|type= RBID
|clé= Pascal:12-0425613
|texte= Electrodeposition of Polymer Nanodots with Controlled Density and Their Reversible Functionalization by Polyhistidine-Tag Proteins
}}
| This area was generated with Dilib version V0.5.77. |  |